Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells

Summary, in English

In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.

• Author: E.J.C. Nilsson; Magnus Gram; J. Nilsson; Jan Pallon; Anna Masternak; J. Paczesny; Natalia Arteaga; Mikael Elfman; Per Kristiansson; Charlotta Nilsson; Bo Åkerström
• Department/s: Infection Medicine (BMC); Nuclear physics
• Publishing year: 2009
• Language: English
• Pages: 1199-1205
• Publication/Series: Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
• Volume: 267
• Issue: 7
• Links: http://dx.doi.org/10.1016/j.nimb.2009.02.051
• Document type: Journal article
• Publisher: Elsevier
• Topic: Subatomic Physics
• Keywords: irradiation; Microbeam; Patterned cell dish; Microdispensing; Cell substrate; Bystander effect; HepG2 cells
• Status: Published
• ISBN/ISSN/Other: ISSN: 0168-583X